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KMID : 0617919890060010031
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Wonkwang Oriental Medicine
1989 Volume.6 No. 1 p.31 ~ p.42
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Studies on the Effects of Cheongkeumkanghwatang on the Pulmonary Edema of the Rat Induced by the Herbicide, Paraquat
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Abstract
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Cheongkeumkanghwatang has been widely used in the diseases of the lungs and bronchi of humans, based on the oriental medicinal references. The treatment effect of it is well known. In the present experiments attempts have been made to evaluate its effects on the pulmonary edema of the albino rat induced by the herbicide, paraquat.
The paraquat, 1,1´-dimethy 1-4, 4´-bipyridilium dichloride(24.5% solution, Dongyang Chemical Co., Seoul), was diluted in physiological saline(1gram in 24ml saline) and injection of the tail vein of the rat(2.94㎎ per 100ｇ body weight). Following the injection of the paraquat, the rats were orally administered with extracts of Cheongkeumkanghwatang(Group A), Cheongkeumkanghwatang plus Oryungsan(Group B), Cheongkeumkanghwatang with Keumeunhwa(Group D), and Cheongkeumkanghwatang plus Oryungsan with Keumeunhwa(Group E), respectively. The extracts were administered 3 times daily for 2 days.
The clinical signs were recorded and the dead rats were necropsied;internal organs were examined, and the lungs were removed and weighed. The rats survived for 48 hours after the injection of the paraquat were killed by decapitation and necropsied as with dead rats.
The results are summarized as follows:
In the preliminary experiments, rats were injected with saline only and administered with the extracts of the 4 drugstuffs 3 times daily for days. The rats were killed and necropsied;the lungs removed and weighed. No differences were found in the weights of the lungs between the groups;no differences were found in the weights of lungs of these rats and these rats and those of lungs of normal, untreated rats.
Following the injection of the paraquat, the rats showed clinical signs of collapse, depression, reduced appetite and a peculiar gait-the pelvis thrust down with the hind limbs thrown outwards. The respiratory rate increased about 6 hours before death;the respiration was slow and deep immediately before death.
The survival rate at 48 hours of the rats injected with the paraquat and administered with drugs was examined;all group except group B showed better survival rats than control.
The respiratory rate per minute of the rats survived at 48 hours was counted;rats of group A showed almost normal respiratory rate, and rats of the other 3 groups and control showed increased respiration.
Lung weights of rats of groups A, B and E were significantly lower than those of control rats. Lung weights of group A rats were not different from those of normal, untreated rats.
Histopathological examination of the lungs revealed severe edema, hemorrhage and broken alveoli in the control group rats. Lungs of group A rats were simialar to those of normal, untreated rats;while lungs of rats of groups B, D and showed moderate edema, hemorrhage and some broken alveoli.
From the adove results it was concluded that Cheongkeumkanghwatang was very effective on the pulmonary edema when it was not mixed with Oryungsan or Keumeunhwa.
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